LOCUS       EY194550                 413 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08c10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194550
VERSION     EY194550.1  GI:159496913
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 748 was extended to value 749 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 652
            PolyA tail found inside read...SR being truncated to 564
            Potential full insert read...vec-to-vec length: 485
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat gtagacctgc ggtcgggtga atgggaagca atcatgcggc aaaaaccgaa
       61 gacgcaaaag tccaagacat ccggtaaaat catagccatc gagattgttg gcgctgatgt
      121 ggtgaaaaac gctgacacag tctgccgatc ctcgaacaga gttgcctcag tggctcaact
      181 ggtcagcgct gagcacgcgg ccgagtacaa gaagcagctg taccgactct gcgaaattcg
      241 ccaaaatatt tgaacgcaag ggagacggac gtattgccgt tgtgaccgtc cgatcgatct
      301 ctaccaatta cacatccacc atcatattta tttgaacctt caatgcgcta ccgaaaacga
      361 tcgattttca tcatcattat tattaaatgt ggaagataaa gagcactcga tta
//