LOCUS       EY194547                 438 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08c07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194547
VERSION     EY194547.1  GI:159496910
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 813 was extended to value 813 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 632
            PolyA tail found inside read...SR being truncated to 589
            Potential full insert read...vec-to-vec length: 510
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ccgtccagca acactggggg aattccgatg cctccgcagg cagcgttcgc
       61 ggcctatcaa gacagcggaa aaggcgatat gaagggcttg agatcggagt tgagcggtgc
      121 atcgacgtcg agggcactgg gcgccgcaaa gactcgcatc gtgtgtccgg acgagctcac
      181 ttcactggag gaactgatgg ccctgcgcgt ggtgccacag ttcgcgcatg ggctgatgcc
      241 ctttgtgcgc taataaccag tcagtggtct ccatcttgtg tccgcgacgc aatgtctcct
      301 tttgcccttt ctgtccccct tttgccgtgc gaggaggtcg tttttgtctg tccaagacgt
      361 cgagtaagtc cctaaagaaa gcgatacaca cgtccgtttg ttgcaatgtc tgaactgatt
      421 acgataaagc aatgacga
//