LOCUS EY194546 352 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab08c06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194546 VERSION EY194546.1 GI:159496909 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 352) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 545 PolyA tail found inside read...SR being truncated to 502 Potential full insert read...vec-to-vec length: 424 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..352 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga agatgtgcgc aatttagaag aggacattcg gctggcgcag cgcatattcg 61 aggtggacac cgagggcgtt cggccgatgt acagcattgt tgaggactac ctgacgtgtc 121 cgttgcgtga ggatgtgccc accgagcagc cattgcaaaa gcacgaggtg ctcgagaacg 181 cggcccagag ctatgaagga tttttcgtca cacccactgt gggagttgtg ccgcccaaga 241 gacaacgcca caaaatggac acgggatccg atagcggtga agtggcttag ccggaagagg 301 caattttaga gattgggaaa ttctccgttt tcaaataaat ctggtttatt ta //