LOCUS EY194545 503 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab08c05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_497763.1| Galectin LEC-4 (32.4 kD) (lec-4) [Caenorhabditis elegans] pir|T19933 hypothetical protein C44F1.3 - Caenorhabditis elegans emb|CAA88850.1| C. elegans LEC-4 protein (corresponding sequence C44F1.3) [Caenorhabditis elegans], mRNA sequence. ACCESSION EY194545 VERSION EY194545.1 GI:159496908 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 503) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 692 PolyA tail found inside read...SR being truncated to 653 Potential full insert read...vec-to-vec length: 575 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..503 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgg cccaaattgc cattttgtgg ccaattcaca gcccagtcca atgagtccag 61 gctgtctttt ctagcttcga gccttgcaag gctatggctc ttcaaacatc ctggcaccca 121 atcgtctgca ttgtcttctc tataatcgcc tttcgctttc cacaggagaa ctgcgtggtc 181 cggaacaact gccaccgtgg ccagtggaac catgaggagc gccacggggg gttcccgttc 241 cgcgcgggcc aaatgttcac gctggacctg agcgcggaga tgaactctgt gcgcgtccgg 301 gtgaacgggg aggaccactg cgagttccag caccgcgaca acttcttcga ggcggaccag 361 ctggaggtgg agggcgacct ggagcagctg cacatggtcg gcgtcaagga cttctaggcg 421 cggcgagaac agcaaaaatg atgtggaact taccatccat tttggattgt gtggtttttt 481 gtctttgata aattgtcgat cta //