LOCUS       EY194542                 480 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08c02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194542
VERSION     EY194542.1  GI:159496874
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 669
            PolyA tail found inside read...SR being truncated to 632
            Potential full insert read...vec-to-vec length: 552
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatt gattaaggag gaggttatga aagtatcaat aacacaggcg gtagactaca
       61 aggtatttag cttagtacag gtgacttgtc agaaaactga ctcgacacct cttttagtcc
      121 ctggtgcttc atcgtgttgt acatattgtt cttgtagttg ctcttgcggt gcatcctcga
      181 ttgatgttga tcataaatta aaggttgtta tagctgatgc ttaaaattat gatccagctt
      241 ggaaataaaa tattattatt tttaataaag tatcatacta gagttttttt atagtcccta
      301 aatctatatt atacatgatt ataaactaat tattgactgt aaaaaatttt tatgttaccg
      361 actataaaaa tttttaggtg tcctaacaag gaggaagttg gtattaatta aatgcagtag
      421 actaatctat ttatttaagg aggaagttat gaaagtgtca ataacacagg cgatagagca
//