LOCUS EY194541 401 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab08c01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194541 VERSION EY194541.1 GI:159496873 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 401) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 590 PolyA tail found inside read...SR being truncated to 552 Potential full insert read...vec-to-vec length: 473 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..401 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtac aagatatcga ggcgctgatg ttactatggt caattttagt cggcaaacac 61 tttaaaatca acgatgtgcc gatgtcgatt ctgactcctc tgaagaataa acggcttgag 121 aacattccac tgaaggaaaa agaacagatg tggctcaccc gttacaccgg acgctacaat 181 aacaaagtac gcggattgca gcgaggcatt cttgaagcgg ttggaaccaa ccaagtcgac 241 tgttgtgtgc tggtgaagat ctatgccacg acgacctttt attcgaaatt gatcatggag 301 aaggttatga ggggaattcc agtcgttttg gaaaactaag gtgttttcga cgatcatttg 361 ttgttgcatt ggttgtggtc ttgttaagcg ttatgttcac a //