LOCUS       EY194531                 350 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08b03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194531
VERSION     EY194531.1  GI:159496839
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 823
            PolyA tail found inside read...SR being truncated to 501
            Potential full insert read...vec-to-vec length: 422
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg tcttaactgt ctacaggaat tgaagcccaa cgctccacta ttcttgcatg
       61 tttaccaaag taccctccca ttttggggtg ataaaaggcg ggaaaggaag aggatgcaga
      121 tgatctcgag agcacctccg cccgcccatt ttcttgcaat ggctgatttt gtcggattta
      181 ctccagccat tgaacaactg ttgagttcac tagatgcaga tgaggaagaa tttccgatta
      241 cgaattcatt aaataagagg gctagaagta cgagtgctat tggcggagac gagaaatatt
      301 aaagatcaga atcaacttta tattatctac gcaataacaa tttaattcga
//