LOCUS       EY194528                 300 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab08a11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194528
VERSION     EY194528.1  GI:159496836
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 494
            PolyA tail found inside read...SR being truncated to 450
            Potential full insert read...vec-to-vec length: 372
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaga gcgactacgc gtggcgacac aaatgaactc gcagacgttg tgcggagtcc
       61 gggccagagt tctcttttct ggtttagggc gccagtccct ggaattggct cagcctgaga
      121 tagggacggc gcgcccgtaa agcaccgcgg ttcctgtggt gtcccaagcg ctgcgcatga
      181 cccttgaaaa tgcgagggaa gcgttgtgaa tctcccgcct gctcgtaccg atatccgcag
      241 caggtctcca aggtgaacag cctctagtcg atagaataat gtaggtaagg gaagtcggca
//