LOCUS       EY194451                 447 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab07a03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194451
VERSION     EY194451.1  GI:159498171
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 640
            PolyA tail found inside read...SR being truncated to 601
            Potential full insert read...vec-to-vec length: 584
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgaggga ccatcttctg ctgtagcgcc agcaaccata aatcagaaca ttaagttgat
       61 tgggttgtgt ggcgagtgta ctacgtgcac ccaaccgaag aatcaacagc gcaatcgcgg
      121 gcatcgatac aacttgcgag tcagagatgt cacctttaca ccgcatcaag cttgaacaga
      181 atgagtcagc ccaatgcccg aattcctttc gacattcatt ccacgttcac tccattcatc
      241 caccataaag tggcctttcc atgcacaatc ttcttgcctc cgtttgttct ccacactcag
      301 ctgttaactt attttgagac atcttaaaat caacgtttcg tttggcaatc gactctcaaa
      361 gtgtaatttg cataatttta ttcgttcttc gcttttcaaa catccacatg tactaaaata
      421 tgcaagacag tataaatttg aaccaca
//