LOCUS       EY194428                 262 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab06f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_776852.1| polymerase (DNA directed), delta 1,
            catalytic subunit 125kDa [Bos taurus] sp|P28339|DPOD_BOVIN DNA
            polymerase delta catalytic subunit pir|A39299 DNA-directed DNA
            polymerase (EC 2.7.7.7) delta catalytic chain - bovine gb|AAA30493,
            mRNA sequence.
ACCESSION   EY194428
VERSION     EY194428.1  GI:159498129
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 466
            PolyA tail found inside read...SR being truncated to 425
            Potential full insert read...vec-to-vec length: 346
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatt gtgccggaac aatgcaaagc gaagtgttct gctcatcccg ggattgtcca
       61 attttctaca tgcgcgaaaa ggtgcgcaat gacctaaaag aagtgcaacg gacacttcga
      121 cgttttcaca taaacaatgg aacgcacaga gggaaagaaa acgcacagga ttgaacgact
      181 acgatatttg atttaataaa cgaggatctc cttttaaaca ttttgacttc gccaatttat
      241 ttgttgcgac ggaacaataa tt
//