LOCUS       EY194412                 442 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab06e07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194412
VERSION     EY194412.1  GI:159498095
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 632
            PolyA tail found inside read...SR being truncated to 594
            Potential full insert read...vec-to-vec length: 514
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcag atacaaatga accacggttc caacttcgcg cggtcactcc tcctcctctc
       61 tcctctcacc gctagttaaa catcgatttg cagtttcaca gcggacccgt tgctttttga
      121 gtcagtcccc tctttgcccc cccccccatc tgtctctatt ctcactcgag ctcaattgta
      181 tcgcttcccc gaaacaactt cgattggtcc acagcacata atctcgatct aaccgtttga
      241 ctggaccctt ctttcccgtt tcccaattat tgtgattcaa tttgtaccag tttctagcaa
      301 atttcattat tcgtcaggat atataataga ggctaacaga gatacctatt actttttgac
      361 ccaacgtcat ttgtaccata tttataggac aacgaaataa ggtagtgcca aatatgtatt
      421 tgtggaataa aaatattacc ga
//