LOCUS EY194409 191 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab06e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194409 VERSION EY194409.1 GI:159498092 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 191) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 805 was extended to value 806 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 386 PolyA tail found inside read...SR being truncated to 345 Potential full insert read...vec-to-vec length: 263 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..191 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg cgagattaca cactggcata atcactttta tcattgtttg gaaattgcag 61 atacctaatt ctatagtagt caagccattt gatattattg tttatttatt aattatacta 121 ttatacataa gtatcaggaa cagaaaacac atttatgcaa attactaaat ataaatagta 181 aaaagttacg a //