LOCUS       EY194405                 295 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab06d12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_492611.1| Elongation factor EF-2 like
            [Caenorhabditis elegans] pir|T23556 hypothetical protein K10C3.5 -
            Caenorhabditis elegans, mRNA sequence.
ACCESSION   EY194405
VERSION     EY194405.1  GI:159498080
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 486
            PolyA tail found inside read...SR being truncated to 446
            Potential full insert read...vec-to-vec length: 367
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca tctgcccgtg gtggagagct tctcgttctg cgacgacttg cgcaaacgga
       61 ccagcggcac cgcgtccgcg cagttggcct tttcgcactg gcaactgatt gaggaggacc
      121 cgcactggca gcccaccacc gaggcggagg tggaggagca cggctcagca gcgacggcag
      181 ccaccgacac caaccaggca cgcgtgtacg tggacgcggt gcgcaagcgc aagggactcg
      241 cgactgagga ggtgatcgtg gccagcgcgg agaagcagcg cacacacaag cgcga
//