LOCUS       EY194400                 443 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab06d05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_499094.1| Vacuolar H-ATPase (28.8 kD)
            [Caenorhabditis elegans] sp|P34462|VATD_CAEEL Probable vacuolar ATP
            synthase subunit D (V-ATPase D subunit) (Vacuolar proton pump D
            subunit) pir|S40985 H+-transporting two-sector ATPase (EC 3.6.3.14)
            ch, mRNA sequence.
ACCESSION   EY194400
VERSION     EY194400.1  GI:159498075
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 517 (,)
            original QR value of 816 was extended to value 817 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 669
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa gaagcggatg gcagaagtgc tcaaagaggc agcgttttca cttgcagagg
       61 ccaaatttac ggctggggat ttctcgcaca tggtggtaca aaatgtgtcc actcaagcgc
      121 agtacactgt tcgaatgaag aaggagaatg ttgtcggtgt gttcctgccc gtgttcgagg
      181 cacacaagaa ggagggttcg gacggcgatg cgtataatct aaccggactg ggcaagggag
      241 gtgcaaatat tgccaagctg aagcgcaatt acaacaaggc agtggagttg ctggttgagt
      301 tggccaccct tcagacatgc ttcatcactc tggaccggca gttcggcggg gggcggcgga
      361 tggcgagagg ggacgatgca cacagcttac agggccgatc gaggccacac aatcatcact
      421 cgtgggcaac tggtgattta aac
//