LOCUS       EY194399                 422 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab06d04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_498012.1| Putative endoplasmic reticulum protein
            of ancient origin (30.9 kD) [Caenorhabditis elegans] pir|S72570
            hypothetical protein C35D10.1n - Caenorhabditis elegans
            gb|AAA62566.1| Hypothetical protein C35D10.10 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY194399
VERSION     EY194399.1  GI:159498074
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 828 was extended to value 828 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 615
            PolyA tail found inside read...SR being truncated to 573
            Potential full insert read...vec-to-vec length: 494
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa tcgagcaaag tgcattattt taaaaatgga actgaactaa aggaagtatt
       61 ggcggaagac aaatcgatag tctgggtgat atgtttcttc gcaacttggt ccgacagttg
      121 ccgatatgtt gccccagttt tttctttgct cagcgaaaag tattccctgg ccaacctccg
      181 ttttggaaag ctcgacgttg gaaagaacaa caaagactcc gagcattttt gcgtcaacaa
      241 tcatcccacc tccagacagc tgccaactgt tgctgttttt aaaagcggtg ttcaacttga
      301 gagaagaccc ctggtggaga atcgccgtgc aatcaatttc gtgttcacag aagaaaactt
      361 gattttagcg ttagagttga atgatcgctt tgcagagtgc aagagaaaaa ttcgttgaat
      421 ga
//