LOCUS       EY194394                 453 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab06c10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194394
VERSION     EY194394.1  GI:159498059
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 647
            PolyA tail found inside read...SR being truncated to 604
            Potential full insert read...vec-to-vec length: 525
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtc gtgatccccg atgacaacgt accattcgag gaattcgcca atcagcgata
       61 tctccaagaa agtctgctgc tgcgacagag agggcgaaaa tctgtcaccc cagtaaaaga
      121 gaagaacgac actgcataca tactcgaggc gtgctacacc gcaccaggag gccgtgaaga
      181 aaggcgccaa attctgtaca caccgcaacg gaactttgac tttgttcgtc gtcgttaagc
      241 gtcggcgcgg tggatccgcc tgagtgggag aactcgcaga gggatatgac ttcttgagat
      301 gtggaccaac ttattccttc atcctgtcca ttcgatgctc aaatgaaaca atccacacat
      361 gttccgattg acactacgtc ttccaaccat cagacctatg ttttcgatat gtaatttaaa
      421 aaaactgtgg aacgataaaa catctcattc aga
//