LOCUS       EY194371                 422 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab06a10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_495207.1| Putative membrane protein, with at
            least 2 transmembrane domains, of bilaterial origin [Caenorhabditis
            elegans] pir|T16732 hypothetical protein R12C12.6 - Caenorhabditis
            elegans gb|AAC46785.1| Hypothetical protein R12C12.6 [Caenor, mRNA
            sequence.
ACCESSION   EY194371
VERSION     EY194371.1  GI:159497645
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 788 was extended to value 789 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 655
            PolyA tail found inside read...SR being truncated to 573
            Potential full insert read...vec-to-vec length: 494
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggct cttgctcaag gtggtagtca tttttgtgat atgtgtgctt ttcatgctcg
       61 gctcttacat gctttatctg gtcgtcgttg acccaatggt cagacggcag cagccattct
      121 tcccctaccg acgtcacgac gacgacacag aggagaacat attcgctcac ccaagcgtgt
      181 ccggcatcga ggagggcaac agtgagagcg aaatccagat gcgagcgcgc cattcgttgc
      241 acgagaaagt gatcagcaag gtggttgccg aacagaacaa gtggaaatcg gaagtggagg
      301 aacagcgtcg gaacgtactc aatcacacaa tgcttaatta aatcgaagcg cgggtgatcg
      361 cagaacacca ttattctatt gcaggataat ctatttgtcg attgactaaa aaacattttt
      421 ga
//