LOCUS EY194366 291 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab06a05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194366 VERSION EY194366.1 GI:159497629 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 291) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 832 PolyA tail found inside read...SR being truncated to 442 Potential full insert read...vec-to-vec length: 363 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..291 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgt gctgaagaag cagagcgcca aatatttgtc catcaatgcc gctctggttg 61 ctgtcttggc aacggctagc gcagttgcgt tctacaacat tgacaagatc tccggttact 121 gggccactcc ctttgtgctc tatactgccg ggatgagcgc cgcggttggc atcaagtcgg 181 ccgaatgaag aagaattgga aagcaactat aattgaatta gaacccaaaa cgccgatatt 241 tggactcgaa acctaaacct atttatcgac tttaaagtac ggctcagttc a //