LOCUS       EY194364                 509 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab06a03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_500884.1| Putative protein [Caenorhabditis
            elegans] pir|T33865 hypothetical protein H04M03.4 - Caenorhabditis
            elegans gb|AAD12787.1| Hypothetical protein H04M03.4
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY194364
VERSION     EY194364.1  GI:159497627
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 806 was extended to value 806 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 698
            PolyA tail found inside read...SR being truncated to 661
            Potential full insert read...vec-to-vec length: 581
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa ttttgcaaga agttcacgtg gctctacttt cccgacccgg aagtgccctt
       61 ctaccgcgtc acgttctttt cgcgctatgg ggacgacgta acgccggacg ccagcaagta
      121 ctggtctgtg atgtgcgagt gcgcacggcc gagtgacgat ccgataagcg aggcagaaat
      181 ccgccgaatg gccattgaag ggcttatcac caagtcgatc atcctgcggg agcagatcgt
      241 caacgagtac tccatcatgc tgccctacgg atacccgatc cccaccattc agagagacgc
      301 agaattggcg cgggcacacg cggcactgga aagccgtcag atctacagtc gtggccggtt
      361 cggcggctgg aagtacgagg tgtccaacca ggaccattgc ttcattcagg gcaaagagct
      421 gtgcgatcgg ctggtgttgg gcgagccgga gaagctctac aagacgctgg ttcccgagaa
      481 gaggggatga ggggcacgca aaaatgtca
//