LOCUS       EY194355                 294 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05h05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194355
VERSION     EY194355.1  GI:159497605
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 853 was extended to value 854 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 488
            PolyA tail found inside read...SR being truncated to 445
            Potential full insert read...vec-to-vec length: 366
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca agaagcgcaa acaaattgtg gagagagcgc agcagatcaa cattgctttg
       61 acgaacggaa atgcccgttt gcgcacagaa gagaatgaat agacgcgcga tgatgattga
      121 aaagggcagg attgtgttgg tttgaatgat ttgttatgct atttcaattg atatatgctt
      181 gttaattaaa cgtgttcaat ttttgactcg ttttagttgt ttctatttat ccgctatttg
      241 ctatatcctt tcaaaatttt tatcgttcgt atatgtcata aaatagagtt ttga
//