LOCUS       EY194347                 448 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194347
VERSION     EY194347.1  GI:159497590
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 874 was extended to value 874 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 640
            PolyA tail found inside read...SR being truncated to 600
            Potential full insert read...vec-to-vec length: 520
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg cgctgggggt gctggtgttc cagatgctgt tcggcttcag ggcgttcccc
       61 tatgaggtga aggcgttcta ctacaggcag gccgcctacc tgaactacca atgggacaag
      121 aaagtcgcct atccgtccaa tgagttgcaa gaactctaca aagcctcgaa tcctgtgcgt
      181 tttgagacgc tgaagcgcgt catcgagaat tgcctgcaat tcgaacctag aaatcgaatg
      241 gacgcagaac agctcaaaga aatgctgcaa atctaacctt tggacaaagt tgtgagtctg
      301 ccgaacccaa ttcattgttt tctccgcaag accacaatta aatatatgca ttgtggtgaa
      361 tctcttccct cactttttcc aagcattttt tgctcgatat ttcgcggaaa gaacgggaaa
      421 agagccgaca acataaaaat attgcgca
//