LOCUS       EY194341                 652 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05f09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_509670.1| Pyridoxal-phosphate dependent enzyme
            family member [Caenorhabditis elegans] pir|T19367 cysteine synthase
            (EC 4.2.99.8) C17G1.7 [similarity] - Caenorhabditis elegans
            emb|CAB01676.1| Hypothetical protein C17G1.7 [Caenorhabditis eleg,
            mRNA sequence.
ACCESSION   EY194341
VERSION     EY194341.1  GI:159497573
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 876 was extended to value 877 (,)
            putative full insert read: vector-to-vector length is 673 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 823
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgc atagccaatg ccaaggcact cgtttttctt cccgttttgg tctgcaaacc
       61 tgcatgggaa tgtaggagtt ggggatctgc ttggccaact cctcggcccg gtccacacag
      121 cccttgatgc ccagcaccgg gtcagtgagc accagctccg ctccgaatgc gcgcaaaagg
      181 gtcctctggg gagaaaccca aaatcatttt aataagtgcg acaaaacagc tcacccgctc
      241 ctcgctcatt gtcgacggca ttgtggcgat gaagcggtac ccgcgggcgg cagaaacaag
      301 ggcgagggct gaattgaaag cggtgcatcc gcgaatcccc gttttaggca gaccgattcc
      361 ggtgtttccg ctggtcggct caatgatcgt gctcacgccc ggcttgagca gtcccttctt
      421 ctcggccgca tccaccatgg aaacgccgat gcggtccttc accgagccgg ccgggttcat
      481 gaactccagt ttgacggctg caaaggcatt ggagaagaaa cacatcggag tgttgccgat
      541 cagactgcac acatctgcgc caatctcgtc gcgggaagcc attttctagt tgtttacagt
      601 ttatctgcac aaattgaaat aaattatttg aatgggacca aaaaaaaaaa aa
//