LOCUS       EY194340                 477 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05f08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194340
VERSION     EY194340.1  GI:159497572
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 671
            PolyA tail found inside read...SR being truncated to 629
            Potential full insert read...vec-to-vec length: 549
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtat ctggttgatc ctgcctgaac tgatgtgttc atttcaaaga ttaagccatg
       61 catgtctaag tataaatcga aacgatgaaa ccgcaaacgg ctcattacac cagcagtaat
      121 ctatttgacc ttgataacct acttggataa ctgtggtaat tctagagcta atacatgcac
      181 aaaaactccc aacgcaagga gggagcgcat ttgttcgcca caaaaccaac ccccctcggg
      241 ggttgagtgt tgaatcagag caactaagct gagcgcacgg tcttgcaccg gcgccgtatc
      301 attcgagctt ctgccctatc aactttcgac ggtagtgtct ctggctaccg tggtggtgac
      361 gggtaacgga ggatcagggt tcgactccgg agaaggggcc tgagaaatgg ccactatgtc
      421 taaggatagc agcaggcgcg caaattaccc actctcagca tgaggaggta gtgacga
//