LOCUS       EY194330                 426 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05e10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_496710.1| DEAD/DEAH box helicase and Helicase
            conserved C-terminal domain and Sec63 domain containing protein
            [Caenorhabditis elegans] sp|Q9U2G0|U520_CAEEL Putative U5 small
            nuclear ribonucleoprotein 200 kDa helicase emb|CAB60351.1|
            Hypothet, mRNA sequence.
ACCESSION   EY194330
VERSION     EY194330.1  GI:159497554
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 855 was extended to value 856 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 620
            PolyA tail found inside read...SR being truncated to 577
            Potential full insert read...vec-to-vec length: 498
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcgg gttccgttct acagtcacca ttacacaccc gagaatctgg aggagggctg
       61 gtacctggtg attggcgatc cagccaacaa ccagttgctc tccatcaaac gcctcaccat
      121 caacacaaag gcacaacacg tgcttgattt cgtccctgcc cagcagtcgt cgaaggccaa
      181 cttcaaactg tacttcatat gcgactccta cctgggcgcc gaccaggaat ttgatctccc
      241 gctgaaaatc caagatgcgc cgagcacttc agacaagtct ggagggcgca aacgcagaca
      301 cgacgactcg aaatggggac gaaacacgtt gatcaatgat caccactccc gctcttgtac
      361 ttctatcaat gtctacaata tttgtttgcg cgaatctagt tccagtttta tataaacgcg
      421 ttgtga
//