LOCUS EY194329 261 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab05e09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_612366.1| hypothetical protein BC010682 [Homo sapiens] gb|AAH34235.1| Similar to hypothetical protein BC010682 [Homo sapiens], mRNA sequence. ACCESSION EY194329 VERSION EY194329.1 GI:159497540 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 261) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 886 was extended to value 887 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 502 PolyA tail found inside read...SR being truncated to 411 Potential full insert read...vec-to-vec length: 333 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..261 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggacc aataatcaat attcaattgc cgtcccgcaa agaagattgt caatttgtat 61 tgcgaccagt gagtgattct gttgggtctt tctgcgatgc attgttgaac gaagattctg 121 ggatagaagt tgtcacattt tactcgccgg atcatatcgg cttttattgg atcgatgata 181 actcttggaa atctatcgat actttgtgtt tctacaattt ttgagcatgt tatttgcctt 241 tggataaaaa taatgccgag a //