LOCUS       EY194324                 252 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAF76926.1|AF159591_1 hypothetical esophageal gland
            cell secretory protein 12 [Heterodera glycines], mRNA sequence.
ACCESSION   EY194324
VERSION     EY194324.1  GI:159497535
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 874 was extended to value 875 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 445
            PolyA tail found inside read...SR being truncated to 402
            Potential full insert read...vec-to-vec length: 324
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ctgtgccgcc aaattctgct ggaggacttg aaggagtcgc gcaaacacga
       61 catcctggag tgggccgaag tgcccgccaa gaccaaagag cagctggaga agcagcagtt
      121 cgacccgctg tgcggcaaag agtgagggga gaggcgaagc aaagagttgg gaccaaaaga
      181 gtgatgattg cctattgcat tggagcaaaa acacttatct atcgactcaa taaacatgaa
      241 ggaaaatcca ca
//