LOCUS EY194321 201 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab05e01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194321 VERSION EY194321.1 GI:159497532 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 201) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 331 (,) original QR value of 880 was extended to value 881 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 481 PolyA tail found inside read...SR being truncated to 353 Potential full insert read...vec-to-vec length: 273 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..201 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga acacggtgct ggcctccgcc ctgttggagg atgggcggag agaactgccg 61 agggatggat ggacgatggg cacctgagaa cgcataacac ccaataaata gtaataatgg 121 gtccgacact gtgatagcca tattcagcat tttcaacatt agtcaaaatg cccatttcca 181 ataaatcaaa acattcaact a //