LOCUS       EY194318                 399 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194318
VERSION     EY194318.1  GI:159496814
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 593
            PolyA tail found inside read...SR being truncated to 550
            Potential full insert read...vec-to-vec length: 471
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatg gagagtcggc tgcgttggaa gtcaaaatgc taaaatccca tatgcggatt
       61 tggccgcaca tgttggcggt tgtagtggca atagtggtgt gccaagttgg aatcgggctc
      121 acactcgcgg caacacttgc ggcaatcgga ttcgcgctcg ttactggtga aacagccaaa
      181 ttgaagaagg aaattgcggg cttggcgagc gaggtcgctc aactgaaacg ctgaggaaga
      241 tgtcggtcga accaaaagaa gtaaggcatt cgacttgccg attgctgttg accaatccga
      301 caattccgat atctcagctt ttatgctatt ttatttggga tctttgactc catcttgtta
      361 cattaaattt ggatataaaa gataaagtga gccttgcca
//