LOCUS       EY194313                 298 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194313
VERSION     EY194313.1  GI:159496809
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 869 was extended to value 870 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 493
            PolyA tail found inside read...SR being truncated to 451
            Potential full insert read...vec-to-vec length: 370
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggctc gacgggcgcg aaattgctgc tccgcggcca ccgctgctgt ttttagtttc
       61 cgttcctgtt cctgtccctt catgtgcggc ctaatagctc ttcggaagtg atggactggc
      121 tttgtggact ggatggactc tgctctgtgg aataggcgca atcaaaccat atttcgggtg
      181 ataaggaagg gaggacagga taaagagcgg tttttgggat tataatgcgg ttgttgttaa
      241 cttgttaagt tatcgttata tcttatatgt acaagaataa agaactaaaa atgcgtta
//