LOCUS       EY194305                 374 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05c06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAC28353.1| putative calponin [Meloidogyne
            incognita], mRNA sequence.
ACCESSION   EY194305
VERSION     EY194305.1  GI:159496775
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 890 was extended to value 891 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 568
            PolyA tail found inside read...SR being truncated to 525
            Potential full insert read...vec-to-vec length: 446
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg agactcgcaa aagctgatga ccggcttcgg tacgccgcgt gacgtgaagg
       61 gcaagcactt gaagcgcatt tgggagctgg agtaccccga ggaggcagaa atctcgctgg
      121 accgcctctg aagaggaggc gactgtcccg cgggcacaaa aaacagaagc ggaatgaaaa
      181 ctgtccacgg aatggcgaac tgtccgtccc ctgtcccctc tctcctcgtg cactgatttg
      241 gccattgcct gacaaccacc aaatatgtcc atccccccta cttctcctcc gctgctatct
      301 attctcctcc tctgaccacc gatcgatgct gaagacttga caacattttg caaatggcga
      361 gcataaacca gtca
//