LOCUS       EY194303                 510 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05c04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194303
VERSION     EY194303.1  GI:159496773
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 883 was extended to value 884 (,)
            putative full insert read: vector-to-vector length is 606 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 754
            PolyA tail found inside read...SR being truncated to 661
            Potential full insert read...vec-to-vec length: 582
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggacg agaagggctg ctacatcgtg ctgaagggcc ccacaaaagc ggcgaacgac
       61 agcgcaaact tttaacggat acccagtcca tttcctccat atcgatgctg tttgtgccct
      121 ttccggtgtt ttaagattcc gagttctaat cttttcccca tcaacaagac cagtcatttt
      181 ttcatttgta catggcagcc ggtttttctg tccgatatta ttgttgtcca gttcggtcac
      241 attcattatt tctgtgttat tattaatatt acgttattag tcagttcgct tgacctttct
      301 cactgcaccg acctcatttc cgtttgttgt gagcacaatc gtgacataaa actccttcgc
      361 cccatttaaa tttcactttg cgaccctttc ccttcaacgc atagtaaact attattttac
      421 tcgaccaagt cacatgtcag agtcccaaaa cttgtcgcac aatattacct aatacctaca
      481 atcttgtgtt gatacaattc ataatattga
//