LOCUS       EY194292                 515 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab05b03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194292
VERSION     EY194292.1  GI:159496731
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 699
            PolyA tail found inside read...SR being truncated to 665
            Potential full insert read...vec-to-vec length: 587
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga agagcggctg aagaagtaca tctcgagaaa ggcggaaagg gagaaggaaa
       61 agcagcggaa gaaggacgag aaaatagaac ggctcaagtc gggcgcgaag accaaacacg
      121 aatttgcaga tcagacctac ttgcggaaga gagacgtgat tctggacgag gtggaagacg
      181 cgattgaagc aggcatgagc gcactccaaa agcacaagga agaggacgtg tgcggagaaa
      241 agctgaaggc cgtatcggca gaggaggagc agcagccgtg ttcttcgaat gacggggatg
      301 gaaataatgg caaagagctg gcacaagact tgggcgcaga aaatcttggc gatgagtcaa
      361 gcggttcgga cgtagatttg gatgacctga tgagcattgg gcctccgaag aagaagaaca
      421 agaaattctg atggacgact gtgtttttaa ttgctctcca cgcaccatca ttaatcgaaa
      481 gggcaaatgt tccgcataaa ttttaatttc gaaga
//