LOCUS EY194282 211 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab05a05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to pir|E81737 hypothetical protein TC0128 [imported] - Chlamydia muridarum (strain Nigg), mRNA sequence. ACCESSION EY194282 VERSION EY194282.1 GI:159496693 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 211) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 871 was extended to value 872 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 406 PolyA tail found inside read...SR being truncated to 362 Potential full insert read...vec-to-vec length: 283 COMM possible reversed clone; protein similarity on positive strand Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..211 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt ataaagagca cttgatggat gccttggcgt taagaggcga tgaaggacgt 61 ggcaggctgc gataagctgc ggggaactgt caacaagttt tgatccgcag atttccgaat 121 ggggaaaccc aactagctta gctagttatt ttatgctgta taagtatgaa aggtaaactt 181 ggtgaactga aatatctcag tagccaaagg a //