LOCUS       EY194275                 459 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab04h08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194275
VERSION     EY194275.1  GI:159496658
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 645
            PolyA tail found inside read...SR being truncated to 610
            Potential full insert read...vec-to-vec length: 577
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg tttggacagt ttcaatgcag cggatggtct tcagcaaatt gcccgggctt
       61 acatgcactt caaatcagat gacgaattga accatggatt tgaaaactgg gacgaataca
      121 aactggaacg cgggagattt cactacattc gtcgctcgat ctactggaac ggctattact
      181 gcagttgcta tttgggatcc aagcgaaaaa tgtgtaagca tgcggtgctc atcatgatca
      241 agaaggggat ggtcgaatat cccgcatcgg tcacagccga actcttgcag ccggcaaaga
      301 agcgtggtcg gcctaagaaa gttgctggag cgcttgttcg tgattaaaaa gtcatatcga
      361 tcgatctcta tacaatttat gcatcacgtg ctatttttta acctattttc gcaaaatgtt
      421 cgttttaccc ataccgatga taaacaaaca actatccca
//