LOCUS       EY194269                 422 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab04g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194269
VERSION     EY194269.1  GI:159496652
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 812 was extended to value 817 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 612
            PolyA tail found inside read...SR being truncated to 573
            Potential full insert read...vec-to-vec length: 494
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggcc aagccagagc agcgaggcca tcgtgcccca atttgcgtgt gctcctcccg
       61 cgtgtaccgc ctcttcactc tcgactggtg aaaacaaatg caacgaggag gcgggaggaa
      121 gtgaagaaat gcgaggaaac tgattaaact ggtcacacac agcgtccgct tgttctaatt
      181 tcatttttgt ttgattttca ccaaatttgt tttgcgtcag ttcttgacag tttttgacac
      241 atctgtgctc atatattcaa caatcatact gtgtatctat tttatattta ttgttagcgc
      301 ttgaaagacc aaatcgtttc cagtttctaa aaagcaaata acccatttcg tattccgacc
      361 gtacatttaa cgcgaaatta gtatgatttt gtacaatcgt tatgtataaa tagcgggtaa
      421 ca
//