LOCUS EY194267 483 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab04g08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_505961.1| RHesus antigen Related RHR-2 (51.0 kD) (rhr-2) [Caenorhabditis elegans] pir|T18673 hypothetical protein B0240.1 - Caenorhabditis elegans emb|CAA98418.1| C. elegans RHR-2 protein (corresponding sequence B0240.1) [Caenorhabditis ele, mRNA sequence. ACCESSION EY194267 VERSION EY194267.1 GI:159496650 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 483) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 795 was extended to value 796 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 700 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..483 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa attaacgcga caattaacct taatgcgatt tatactaaat attattttcc 61 cgctcatcac tcatcagcgt cgtcaattct cggtgcacgc caactacccc atgttccagg 121 acatccacac aatggtgatc atcggattcg gctttctgct ctccttcctc aaaaaatacg 181 gcttctcggc cctgtccatc aatctgctgc tctgctcctt cgtcatgcag tacgcactcc 241 tgctgcgcgg cttcctctcg cccgggttca tccggacggg ggtgtgcacc atctccattg 301 acgagtgagt ggtgacctgg tgactctggc atgggaccgg aaccgctacc aaacgggcca 361 ccaaacccca cattttgctc tgcagtaact tctgtaataa tgcaaatgca aatttgcttt 421 caaagacaaa aaatggtgct aaaaattatc ttttcaatga gataaggcat ttttgataat 481 tgg //