LOCUS       EY194264                 219 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab04g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194264
VERSION     EY194264.1  GI:159496617
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 767 was extended to value 772 (,)
            putative full insert read: vector-to-vector length is 367 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 514
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac ctcggacgag ggcaagacgg cgctgaaggg cttcccgcgt ctgttggtgg
       61 acgacatgtt ccagctgggc cgcaactacg tacaccaggg cgtctacgtg gcagtggact
      121 tcttcaacag atagaagcag catagctgaa agtaattgat ctgatgcggt gattcaaaag
      181 cacacaccga gtgatcccca aacaaatggc ccaatcact
//