LOCUS       EY194260                 503 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab04g01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAO49799.1| arginine kinase [Heterodera glycines],
            mRNA sequence.
ACCESSION   EY194260
VERSION     EY194260.1  GI:159496613
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 692
            PolyA tail found inside read...SR being truncated to 654
            Potential full insert read...vec-to-vec length: 575
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg cgtgaaagca atcgaatcca aagtaccatt ttcacgtgat gaccgactcg
       61 gctggcttac gttctgtccg actaatctgg gcacgactgt acgtgctagt gtgcacataa
      121 aattgccaaa gattagtgca aagcctgatt tcaaaaccat ttgcgacggt ctcaagctac
      181 aaattcgcgg aattcacgga gaacactcag agagtgctgg tggagtctac gacatctcca
      241 acaaagctcg acttggtcta actgaatttg aggccgtcaa gcaaatgtac gacggagtca
      301 aacatctgat cgagcttgaa aaggccgcct aaggaattga atcgcgatta gacggacatc
      361 attgacttta atcatagtta tagcaattta tgtaattttt tcctgatggc gatcgagaaa
      421 gagacatacc agaatccgaa tattgaattt gaataatttt tcatattata gtttggctgg
      481 ctttaagcat aaattgattt tca
//