LOCUS       EY194255                 332 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab04f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194255
VERSION     EY194255.1  GI:159496579
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 773 was extended to value 773 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 524
            PolyA tail found inside read...SR being truncated to 483
            Potential full insert read...vec-to-vec length: 404
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtcg tggagagcag ggaactgcag gacatgacca ccacgacgtt ctcgaacccg
       61 gtctacgacc acggcatcga cacgctgagc tacaacgacg acgcgggttc gaccaagttc
      121 gagacgattg aaacgggcaa aagtaaccca gtgtacgagg ctcaccactg acaatttgca
      181 caaatgtgat cgccgactgt tgaacaaagt gtattcccaa actgtactta gcaaaagggt
      241 caaagtgcct ctctcctgca caaccaaatg cacttataaa tgtgattctt cgccctcacc
      301 tattgtttct ttttgcgctg ataaacatgt ga
//