LOCUS EY194254 453 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab04f06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_496565.1| ATP-NAD kinase [Caenorhabditis elegans] pir|T26501 hypothetical protein Y17G7B.10a - Caenorhabditis elegans emb|CAA19455.1| Hypothetical protein Y17G7B.10a [Caenorhabditis elegans], mRNA sequence. ACCESSION EY194254 VERSION EY194254.1 GI:159496578 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 453) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 832 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..453 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtg ttcatcgggg agagccatgc cgcacgggct cacctattac gacgtgctag 61 tggactgcgg gccaatgacc aaacagaaga gctccggaat gaccatttgc actgggaccg 121 gctcatcttc gtggcactac tacatcaacc ggttgacgga gcataatctc agcaaaattt 181 tcaccgcatt gacgcagctg ggcatcaatc tggaccgcga gatggacagc gagctgatcg 241 aggagatttg tcggcggaac aacgagaagc tcaaattcag tcctgagctg caaatgatgg 301 cgttcggtgt tcgcgatgcg gtgttcaacg cctcatttcc gatgacgccg atccggggat 361 ttgcaaacaa aattatgtta aagagccgat gtatgcacgc gcacctcatc ttggacggct 421 ccagctcgat cccgttcaac cgcggcgcag aag //