LOCUS       EY194251                 446 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab04f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194251
VERSION     EY194251.1  GI:159496575
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 821 was extended to value 822 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 635
            PolyA tail found inside read...SR being truncated to 598
            Potential full insert read...vec-to-vec length: 518
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac aataaaactg cccattctct ctccctgttc tcgcctcact aattcccatt
       61 cttttttgga aatgtctctg atcggagttc cgctggcgtt gctcgtctca gtggctttgg
      121 tcgcattgct gctccttttc ggcgcattct tgttccgccg tgtgcccgag aagaagcgga
      181 agaatgtcca gaagaaggcg cgagtgggag agcaagcgca acaacaagaa gcggaagggc
      241 gagaggaacg gccgccaatt gaacgagtgg cggacttcca cgaaatccca ctgggagaga
      301 gggacaaaac gacggatgga cggccgccca ggattgtgct gaccaccgcc tcaatgaaaa
      361 tacacccact ctgactggtg tgtttggcag ccgagaatag ccataaaatg taaaagtcta
      421 ttgcgaaaat aaataataaa tggcta
//