LOCUS       EY194249                 383 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab04f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194249
VERSION     EY194249.1  GI:159496573
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 576
            PolyA tail found inside read...SR being truncated to 535
            Potential full insert read...vec-to-vec length: 455
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggcg aacacgcgga ggtgaacagg gaacccccgt gaatccaccc gaacatcccc
       61 cgaaattggc agctgcttcg gagcagcagt ggccttgcga gtgggctcag cgagaacacg
      121 cgaatgctga gtgctgctgt gcagaacgcg aggagaaagt ccgcgtcaag tcgctacagt
      181 tccagtccgg ccaagcaatt gcctttggag cgtcctgtga tttcattcgc gatgattgct
      241 gacagcgaaa tcgaaactgt ttgatatgca aaaagaatgg tcaaaaaagt cgccaatgga
      301 acaatcgaat ggaagttcac tgtttactat gtgttttcct ttttcatgag cccaaaatct
      361 tgaactaggt tggtgcaaaa gca
//