LOCUS EY194247 360 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab04e10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_594438.1| 60S ribosomal protein L34 [Schizosaccharomyces pombe] sp|O42846|RL34_SCHPO 60S ribosomal protein L34 pir|T38228 60S ribosomal protein L34 - fission yeast (Schizosaccharomyces pombe) emb|CAA16982.1| SPAC23A1.08c [Schizosaccharomyc, mRNA sequence. ACCESSION EY194247 VERSION EY194247.1 GI:159496542 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 360) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 781 was extended to value 782 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 604 PolyA tail found inside read...SR being truncated to 511 Potential full insert read...vec-to-vec length: 432 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..360 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggaag accaaggtgg tcaagacacc aggaggccgc aatgtgttac actatcgcaa 61 taagctcggc tccgtgccca agtgtggcga cactggcgtc ccgctgaaag gaatccaagc 121 tgcgcgacct cgccaattgg ccaagctgac taagcgaaag aagaccgtca cgcgtgcata 181 cggaggatgc ctgagcgctg cagcagtgcg ccagcgaata atccgcgcat tcttggtcga 241 ggaacagcag attgcgaaca agatcctgaa agccaagagt gctggaagga agtaaaccga 301 tggaaggttt tggttccacc atgttctatt tgttgttgca ttaaagtttg ttctgtccca //