LOCUS       EY194245                 449 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab04e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194245
VERSION     EY194245.1  GI:159496540
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 638
            PolyA tail found inside read...SR being truncated to 600
            Potential full insert read...vec-to-vec length: 521
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaca actcgttcaa aaactccatc atcaactgca tcaatggact aatcggcaat
       61 aacccgaccg tggaggactg ctgcatctac tacatcaacg gattataccc atccaacggg
      121 ggcagccaaa ccctcgccat gcgcaaaacg ttcctcgcga cgccgttctt cacctattac
      181 acgcaggaga ctacagtgag catccagatc gcgactatta gcatcttctg tggctgcaat
      241 tgaggcgcaa gacagaagag agtggcgtcc aacggattcc tttctcgagg aagtcaccac
      301 gtgttgagcc gcaccttttc tctccagcac tttcctgtgc atagtcctaa aagaggcaag
      361 acatcgccct ttgacccaaa acgacagaac accgccgctt tttgtagttt tcttacactt
      421 tccttgtgaa taaatggtgc gtgagttta
//