LOCUS EY194241 77 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab04e03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194241 VERSION EY194241.1 GI:159496536 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 77) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 626 was extended to value 626 (,) putative full insert read: vector-to-vector length is 231 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 380 PolyA tail found inside read...SR being truncated to 228 Potential full insert read...vec-to-vec length: 149 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..77 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtca tgtgctgatt ctattatacc tgtatcaaac gatattacac caacatggat 61 ataaataaat taaaaca //