LOCUS EY194172 576 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194172 VERSION EY194172.1 GI:159498371 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 576) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 823 was extended to value 824 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 826 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..576 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtgg ctggaagtgg ccaacactga atggaacaaa tttacagacc gctctgtagt 61 cgaatcaggg cgctatgctt acaaaataat cgctaaaatg cctggcgtaa ttgtaagtga 121 agcgagtgct caaagcgaag aggtgttcat ttctgcaaaa aagagagaag atgcaaatga 181 ttctgtacga acgatcgaaa cggaaccaca catcgagatc aagccaatgc ccacgttgcg 241 tgtggagaga aacgactcga cagattcgtc atccatgtct tcatccgtgc cttccggact 301 tgattctggc atccaagatg atgatgacta ccgcgcaatg atggaatcac cgggagctgc 361 gtcagctggc gctaggaaga aggcactgaa gaaaaaagtg tttataaatc aaaacgatga 421 agcaacagaa ccaatggaac gagtagagga aaagtctaag gagggatcgg aaacaccggc 481 tcaggaatcg aacaacgaaa tgtcgagcac gaaggttcgg gatgaagagc caaaggcgat 541 ggaaacggag accaaagtgg aggaggcgag ggcacg //