LOCUS EY194170 600 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194170 VERSION EY194170.1 GI:159498369 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 600) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 745 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..600 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggcg gctggacagc agcagacagc acagaaacag gcaacgcagc agcaggcgca 61 aaaacaaacg attaatccgg atttacgcaa actggcaatg caaatcggga acgattggac 121 gtcgctgggc caattcagct tcgacatgct cgggctcggg acatcggggc aacaaccgca 181 gcagcagcaa caaaagccgt agacagaaga aaccgagagt tgccagccag atccgaagga 241 ttgcccaata atttattctt tttatgtgac agtcttcgtc gtaatactgt tttgtacata 301 tattatgtat gaatcgggtt tatttttcgt tcaccacatc acttgtattt ggcccttcgc 361 acacagcttg gcaatcgatg tgtctgcgcg ttctttcacg tttggtatgt gcggctgtcg 421 ctcctctggc tgcctgtctc cctcgttcta tctatgctcc gtttctcgtc tcccacctcc 481 attccgggga gtgccacggc agcgtcaata gaggggaggg cgaaaagggc ctcccagctt 541 ggtggctttc ccgcttcgtt ggggtccagt agaagagtaa agctttgatg gcaaaaaaaa //