LOCUS EY194168 540 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194168 VERSION EY194168.1 GI:159498367 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 540) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 593 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 743 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..540 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc cagaccgcac cggccacaat atgtgcccgc ccgcaataca actataatca 61 atagcaacag aactttgtct caccacacca tcagctccca tatacaccag gatatgtcgt 121 cccgacaaca cagtccatct cgcaccgcta tcaactgatg cgaccgcaaa tgtcgaagcg 181 ctacagctcg accaattccg tcggcacttc acttgtggtg caaactttgg cccaactacg 241 tgaacagcac aagaacaaaa cgggactgct tcaaaagaga ttggaggagc agcgaatgtt 301 gttgagccag ctccagaaaa taccgggggc ggatcgtcgt cgcgatttga tgtttcgagt 361 acgcaaactg gatgcaccta ttgcccagtt gaaaggagac ctgacatcgc ttaacgaaca 421 aatcacgacg gtgacgaaaa aatgaagtct gaacttgaaa accgacgtgg aatctctggc 481 attgttctgt cgaaatatct tagttgtacc ctcaacttga ttaatcagag aatttatttt //