LOCUS EY194167 553 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03e10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194167 VERSION EY194167.1 GI:159498366 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 553) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 728 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..553 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtcc tcgaaaacga tcgcggccca caagcaatgg gcgaattcgt gatggagttt 61 gacgagctgg ttcggaacac acaacaactc agcgagtcgg tgaaggagaa ggccgccagt 121 ttggtgaagt tgcgagacaa caaatggggc gttgtagccg agtcgagtgc caacggtctc 181 ccaatcggag tgggcattat tggaccggat ggcgagccgt tgctcactga ggacgagtgt 241 gcttttctgg acgagtcgga cgtatttgga aactccccat cgggatcttt gtacaacaat 301 ctgctggaag aagaccaagt tgaggatgac tacgaacagt tcctccaggc aacagcgatt 361 cgtgccgcgg aaaaggcatt agagaaggtg tcaattgatg atgacgatgt ggagagtgtc 421 tcttcgccga aattcatggc gagtaatgcc aagcaagaat gatgattccc gagcaatctt 481 cgattgtgtt tgatatggaa tgaagattgc atcaattcag ataaacatgt tcaaactgta 541 aaaaaaaaaa aaa //