LOCUS EY194166 532 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03e09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194166 VERSION EY194166.1 GI:159498365 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 532) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 799 was extended to value 800 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 808 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..532 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtcc gatcgattgt gcccctccgc cggcattcgt tcccctctct gcaccaccgc 61 cccacatcgg cacttctctc gttgcacttc catccacggc actgaacagc cccaactgat 121 gagcacctcc tctccttccc tcgcactgca ctcactcccc ccccccaagc acacagcact 181 ctccggacac actgagcacc ctcttgccct ttgctcttcc acagcactcc ccccccccac 241 tcacctctcc tcactttggc gcctctggag cacgtctggc cgcacccacc gaatgcttta 301 ccacaaccac cgcactcccc gtcccaccgc tgtagcttta ttaattggta ttatttatta 361 cttattagag tagccccaca ttttcacttc cactgcactc acaattcttc acacagacac 421 aatcctccac ctcacggaca cagccgctca cttttgcaca aatgcattag acaaaagcac 481 aaatcatggc aatcagaatc agaataatat tagcaattga catttgcatg aa //